What is Direct gene transfer? - Cuntorio

Genes may be transiently or permanently introduced into cultured eukaryotic transfer cells without the use of a vector in the strict sense. A eukaryotic gene on a bacterial plasmid, for example, may transiently express its product when transfected into a cell line, even if the plasmid does not replicate in that type of cell. Alternatively, DNA introduced by transfection or microinjection may